PA5-21315

antibody from Invitrogen Antibodies

Targeting: FANCC FA3, FAC, FACC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21315

Provider

Invitrogen Antibodies

Product name

FANCC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3.

Concentration

0.99 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FANCC Polyclonal Antibody (Product # PA5-21315). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. FANCC Polyclonal Antibody. FANCC Polyclonal Antibody (Product # PA5-21315) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FANCC Polyclonal Antibody (Product # PA5-21315). HeLa whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FANCC Polyclonal Antibody (Product # PA5-21315) diluted at 1:20,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FANCC Polyclonal Antibody detects FANCC protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5 % SDS-PAGE, and blotted with FANCC Polyclonal Antibody (Product # PA5-21315) diluted by 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FANCC in paraformaldehyde-fixed HeLa cells using a FANCC polyclonal antibody (Product # PA5-21315) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FANCC antibody (Product # PA5-21315) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using FANCC (Product # PA5-21315) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.