Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 681002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681002, RRID:AB_2566442
- Product name
- Purified anti-TAL1
- Antibody type
- Monoclonal
- Antigen
- Partial human TAL1 recombinant protein (1-263 a.a.) expressed in E. coli.
- Description
- TAL1, also known as SCL, is a class I basic helix-loop-helix (bHLH) transcription factor. TAL1 is essential for the establishment of hematopoietic stem cells during embryogenesis, and regulating proliferation and differentiation of multiple hematopoeitic cell lineages, such as erythrocytes, megakaryocytes, mast cells, and eosinoohils. Tal1-null mice are embryonic lethal due to the failure of yolk sac hematopoiesis and vascular defects. In hematopoietic cells, TAL1 forms a multiprotein complex with other transcription factors, which include the E protein TCF3, LMO2, LDB1, and GATA family members. The large protein complex containing TAL1 binds to specific cis-regulatory elements encoding the consensus sequence AACAGATGGT and regulates expression of target genes. TAL1 is not expressed in normal lymphoid cells, but ectopic expression of TAL1 in T-cells leads to T-cell acute lymphocytic leukemia (T-ALL).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4G8C07
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human Jurkat cells (lane 1), 293T cells (lane 2), and mouse thymus (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TAL1 (clone 4G8C07) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
- Conjugate
- Unconjugated