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Immunocytochemistry
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoelectron microscopy [1]
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- Product number
- 681004 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681004, RRID:AB_2572200
- Product name
- Alexa Fluor® 594 anti-TAL1
- Antibody type
- Monoclonal
- Antigen
- Partial human TAL1 recombinant protein (1-263 a.a.) expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 4G8C07
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Jurkat cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1 µg/ml Alexa Fluor® 594 anti-human TAL1 (clone 4G8C07) antibody (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by a 60X objective.
- Conjugate
- Yellow dye
