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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9101-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAL-1 Monoclonal Antibody (2TL242), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 2TL242 recognizes human TAL-1. The TAL-1 protein is encoded by the tal-1 (T-acute leukemia or stem cell leukemia (SCL)) gene and functions as a 42 kDa basic helix loop helix transcription factor involved in mediating cell growth and differentation. Rearrangements of the tal-1 gene are common in human T-cell acute leukemias (T-ALLs) and result in aberrant transcription in leukemic cells. TAL-1 expression in leukemic cells is localized to the nucleus although both nuclear and cytoplasmic expression has been shown in normal cells such as erythroid precursors, megakaryocytes, endothelial cells, and macrophages scattered throughout tissues such as the tonsil, cerebellum, kidney, lung and spleen. TAL-1 is known to regulate both embryonic and adult hematopoiesis and more recently was found to regulate proliferation and differentiation of common myeloid precursors to monocytes and ultimately macrophages within tissue. Applications Reported: This 2TL242 antibody has been reported for use in western blotting, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 2TL242 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval at less than or equal to 20 µg/mL. This 2TL242 antibody has also been tested by western blot of reduced Jurkat cell lysate at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2TL242
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references The TAL1/SCL transcription factor regulates cell cycle progression and proliferation in differentiating murine bone marrow monocyte precursors.
An immunohistochemical study of TAL-1 protein expression in leukaemias and lymphomas with a novel monoclonal antibody, 2TL 242.
Expression of TAL-1 proteins in human tissues.
Dey S, Curtis DJ, Jane SM, Brandt SJ
Molecular and cellular biology 2010 May;30(9):2181-92
Molecular and cellular biology 2010 May;30(9):2181-92
An immunohistochemical study of TAL-1 protein expression in leukaemias and lymphomas with a novel monoclonal antibody, 2TL 242.
Chetty R, Pulford K, Jones M, Mathieu-Mahul D, Close P, Hussein S, Pallesen G, Ralfkiaer E, Stein H, Gatter K, Mason D
The Journal of pathology 1996 Mar;178(3):311-5
The Journal of pathology 1996 Mar;178(3):311-5
Expression of TAL-1 proteins in human tissues.
Pulford K, Lecointe N, Leroy-Viard K, Jones M, Mathieu-Mahul D, Mason DY
Blood 1995 Feb 1;85(3):675-84
Blood 1995 Feb 1;85(3):675-84
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum stained with 20 µg/mL Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 20 µg/mL Anti-Human TAL-1 Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
