14-9101-82

antibody from Invitrogen Antibodies
Targeting: TAL1 bHLHa17, SCL, TCL5
Provider product page for 14-9101-82
Western blot
Immunohistochemistry

Antibody data

Product number
14-9101-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
TAL-1 Monoclonal Antibody (2TL242), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody 2TL242 recognizes human TAL-1. The TAL-1 protein is encoded by the tal-1 (T-acute leukemia or stem cell leukemia (SCL)) gene and functions as a 42 kDa basic helix loop helix transcription factor involved in mediating cell growth and differentation. Rearrangements of the tal-1 gene are common in human T-cell acute leukemias (T-ALLs) and result in aberrant transcription in leukemic cells. TAL-1 expression in leukemic cells is localized to the nucleus although both nuclear and cytoplasmic expression has been shown in normal cells such as erythroid precursors, megakaryocytes, endothelial cells, and macrophages scattered throughout tissues such as the tonsil, cerebellum, kidney, lung and spleen. TAL-1 is known to regulate both embryonic and adult hematopoiesis and more recently was found to regulate proliferation and differentiation of common myeloid precursors to monocytes and ultimately macrophages within tissue. Applications Reported: This 2TL242 antibody has been reported for use in western blotting, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 2TL242 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval at less than or equal to 20 µg/mL. This 2TL242 antibody has also been tested by western blot of reduced Jurkat cell lysate at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
2TL242
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
TAL1 protein structure - 14-9101-82 shown in red.