- PA5-30586 - Invitrogen Antibodies TAL1 antibody

Banerjee A, Tripathi A, Duggal S, Banerjee A, Vrati S
Scientific reports 2020 Nov 11;10(1):19587

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of TAL1 using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TAL1 polyclonal antibody (Product # PA5-30586) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using TAL1 Polyclonal Antibody (Product # PA5-30586). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 Polyclonal Antibody (Product # PA5-30586) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: TAL1 antibody immunoprecipitates TAL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TAL1 protein by 2 µg of TAL1 antibody (Product # PA5-30586) 12% SDS-PAGE The immunoprecipitated TAL1 protein was detected by TAL1 antibody (Product # PA5-30586) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Notch signaling in DENV-infected MEG-01 cells. Naive MEG-01 cells (control) were treated with PMA (Mock + PMA), or DENV-infected (MOI-1) cells were treated with PMA (DENV + PMA). Cells were harvested at day 5 pi for isolating total RNA and preparation of cell lysate. ( a ) Expression of Notch-1 transcript relative to Gapdh expression is presented from 3 independent experiments. ( b ) The cell lysate was Western blotted for different proteins (left panel) and the band intensities were quantified by ImageJ software. Intensities from 3 or more blots were determined compared to GAPDH, and average intensities in DENV + PMA compared to Mock + PMA treatment were plotted to show the fold-change in protein expression (right panel). Intensities from 3 or more blots were determined. ( c ) Nuclear (N) and cytoplasmic (C) extracts of MEG-01 cells were prepared and Western blotted with TAL-1 antibody. Levels of Actin and Histone proteins were checked to demonstrate the quality of protein fractionation. Statistical analyses were done using the one-way analysis of variance (ANOVA). * p < 0.05, ** p < 0.005, NS denotes difference not-significant.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Interaction of Tal-1 and DENV E protein. Naive MEG-01 cells (control) were treated with PMA (Mock + PMA), or DENV-infected (MOI-1) cells were treated with PMA (DENV + PMA). ( a ) Cells were harvested at day 5 pi for preparation of cell lysate. Immunoprecipitation was carried out using the rabbit anti-TAL-1 antibody, or the rabbit IgG as the isotype control, and the Protein A/G magnetic beads. The precipitated proteins and the cell lysate used for the immunoprecipitation were Western blotted with antibodies shown on the right side of the figure. ( b ) On day 5 pi, cells were harvested, fixed in 2% paraformaldehyde, followed by permeabilization with 0.03% Triton-X. This was followed by incubation with TAL-1, and DENV E antibodies and stained with the fluorescent-labeled secondary antibody. Cells were mounted on slides using ProLong Gold anti-fade reagent with DAPI and images taken using a confocal microscope. Untreated MEG-01 cells were used as a control. Experiments were performed 3 times and representative confocal images are shown. The right-most panels are zoomed up images of the insets in the adjacent panels. The scale bar size is 5 mum.

PA5-30586