PA5-109638

antibody from Invitrogen Antibodies

Targeting: DLST DLTS, KGD2, OGDC-E2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109638

Provider

Invitrogen Antibodies

Product name

DLST Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: PAKAKPAEAP AAAAPKAEPT AAAVPPPAAP IPTQMPPVPS PSQPPSGKPV SAVKPTVAPP LAEPGAGKGL RSEHREKMNR MRQRIAQRLK EAQNTCAMLT T

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.93 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DLST in extracts of various cell lines. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DLST in U-2 OS cells. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DLST in paraffin-embedded human esophageal cancer. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DLST in paraffin-embedded human gastric cancer. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DLST in paraffin-embedded human liver damage. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DLST in paraffin-embedded human esophageal cancer. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DLST in paraffin-embedded human gastric cancer. Samples were incubated with DLST Polyclonal antibody (Product # PA5-109638) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.