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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLST Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431. Predicted reactivity: Mouse (95%), Rat (96%), Zebrafish (87%), Xenopus laevis (90%), Pig (95%), Chicken (91%), Rhesus Monkey (98%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DLST using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a DLST polyclonal antibody (Product # PA5-22239) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DLST Polyclonal Antibody (Product # PA5-22239). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLST Polyclonal Antibody (Product # PA5-22239) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DLST in methanol-fixed A431 cells using a DLST polyclonal antibody (Product # PA5-22239) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DLST (Product # PA5-22239) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using DLST (Product # PA5-22239) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
