GTX100519
antibody from GeneTex
Targeting: HMGA2
BABL, HMGIC, LIPO
Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- GTX100519 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100519, RRID:AB_1950498
- Product name
- HMGA2 antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Tetrac downregulates β-catenin and HMGA2 to promote the effect of resveratrol in colon cancer.
The long noncoding RNA HULC promotes liver cancer by increasing the expression of the HMGA2 oncogene via sequestration of the microRNA-186.
Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells.
HMGA2 promotes vasculogenic mimicry and tumor aggressiveness by upregulating Twist1 in gastric carcinoma.
The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.
Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells.
MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3'UTR.
The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.
Nana AW, Chin YT, Lin CY, Ho Y, Bennett JA, Shih YJ, Chen YR, Changou CA, Pedersen JZ, Incerpi S, Liu LF, Whang-Peng J, Fu E, Li WS, Mousa SA, Lin HY, Davis PJ
Endocrine-related cancer 2018 Mar;25(3):279-293
Endocrine-related cancer 2018 Mar;25(3):279-293
The long noncoding RNA HULC promotes liver cancer by increasing the expression of the HMGA2 oncogene via sequestration of the microRNA-186.
Wang Y, Chen F, Zhao M, Yang Z, Li J, Zhang S, Zhang W, Ye L, Zhang X
The Journal of biological chemistry 2017 Sep 15;292(37):15395-15407
The Journal of biological chemistry 2017 Sep 15;292(37):15395-15407
Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells.
Liu Y, Fu QZ, Pu L, Song LL, Wang YY, Liu J, Wang ZL, Wang ZM
Molecular medicine reports 2017 Oct;16(4):5107-5112
Molecular medicine reports 2017 Oct;16(4):5107-5112
HMGA2 promotes vasculogenic mimicry and tumor aggressiveness by upregulating Twist1 in gastric carcinoma.
Sun J, Sun B, Sun R, Zhu D, Zhao X, Zhang Y, Dong X, Che N, Li J, Liu F, Zhao N, Wang Y, Zhang D
Scientific reports 2017 May 22;7(1):2229
Scientific reports 2017 May 22;7(1):2229
The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.
Wang Y, Chen F, Yang Z, Zhao M, Zhang S, Gao Y, Feng J, Yang G, Zhang W, Ye L, Zhang X
Scientific reports 2017 May 18;7(1):2070
Scientific reports 2017 May 18;7(1):2070
Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells.
de Vasconcellos JF, Byrnes C, Lee YT, Allwardt JM, Kaushal M, Rabel A, Miller JL
Journal of translational medicine 2017 Aug 2;15(1):169
Journal of translational medicine 2017 Aug 2;15(1):169
MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3'UTR.
Wang Y, Chen F, Zhao M, Yang Z, Zhang S, Ye L, Gao H, Zhang X
Biochemical and biophysical research communications 2016 Nov 18;480(3):455-460
Biochemical and biophysical research communications 2016 Nov 18;480(3):455-460
The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.
Lee SH, Cho S, Kim MS, Choi K, Cho JY, Gwak HS, Kim YJ, Yoo H, Lee SH, Park JB, Kim JH
Biochimica et biophysica acta 2014 May;1839(5):374-86
Biochimica et biophysica acta 2014 May;1839(5):374-86
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 15% SDS PAGE GTX100519 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 15 % SDS-PAGE, and blotted with HMGA2 antibody (GTX100519) diluted by 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted by 1:10000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody (GTX100519) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 £gg of HMGA2 antibody (GTX100519).Western blot analysis was performed using HMGA2 antibody (GTX100519).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human HuCCT1 xenograft . HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HMGA2 antibody (GTX100519) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. HMGA2 antibody (GTX100519) diluted at 1:500.