PA5-21320

antibody from Invitrogen Antibodies

Targeting: HMGA2 BABL, HMGIC, LIPO

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21320

Provider

Invitrogen Antibodies

Product name

HMGA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (95%), Rat (92%), Zebrafish (85%), Xenopus laevis (80%), Chicken (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.08 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma.

Xu L, Ma Y, Zhang H, Lu QJ, Yang L, Jiang GN, Liao WL
Cell death & disease 2020 Jul 27;11(7):593

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody (Product # PA5-21320) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 The oncogenic effects of circASPH in vitro and in vivo. a The morphologic changes in A549 and PC9 cells overexpressing circASPH were observed using a scanning electron microscopy assay. b , c DNA synthesis assessed using an EdU assay in the lenti-circASPH cells and control cells. Nuclei were stained with Hoechst 33342 (blue). The quantified data are presented as the percentage of EdU-incorporated cells. Log2 transformation was applied to deal with the ratio results and to obtain the normally distributed data. Then, T test was used to compare the difference between groups. d , e The migration of A549 and PC9 cells was significantly increased by circASPH overexpression. The migration distances (mum) of A549 and PC9 cells were quantified. f , g The invasion of A549 and PC9 cells was apparently increased by circASPH overexpression. The quantified data are presented as the number of invading cells per HPF. HPF high-power field. h - j Xenograft tumor models show that tumors grown from the circASPH-overexpressing cells were bigger than those grown from the control cells. k Western blot analysis of the levels of HMGA2 protein in the xenograft tumors. l IHC staining of the HMGA2 protein in the xenograft tumors. Every cell test was carried out in triplicate, and n = 3 for each cell group.