Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B6867 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ ANGPT1 / Angiopoietin-1 Antibody (aa76-85) LS-B6867
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Canine, Horse, Rabbit, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with ANGPT1 antibody (1 ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with ANGPT1 antibody (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-ANGPT1 / Angiopoietin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.