Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- AP07808SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP07808SU-N, RRID:AB_1611528
- Product name
- anti Angiopoietin-1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic Peptide corresponding to a region (aa 21-40) near the N-terminus of Mouse angiopoietin 1 protein conjugated to KLH using maleimide. Epitope: N-terminus.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 50 µl
- Concentration
- 85 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot against supernatants of Mouse Angiopoietin-expressing endothelial cells using Angiopoietin-1 antibody Cat.-No AP07808SU-N at a 1/500 dilution to detect Mouse Ang-1. Lane 1: Wt endothelial cells. Lane 2: Mouse Ang-1 (clone 1-8) expressing cells. Lane 3: Mouse Ang-1 (clone 1-15) expressing cells.Lane 4: Mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1/5000 dilution of HRP conjugated Goat anti Rabbit IgG preceded color development.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded Human Lung Vessel stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded Mouse Liver stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded Mouse Kidney stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval.