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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB8220 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Angiopoietin-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Angiopoietin-1 in ELISA and Western Blot. In ELISA, no cross-reactivity with recombinant mouse Angiopoietin-2 is detected.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O08538- Isotype
- IgG
- Antibody clone number
- 892732
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A multifunctional bioactive material that stimulates osteogenesis and promotes the vascularization bone marrow stem cells and their resistance to bacterial infection.
Ma C, Wei Q, Cao B, Cheng X, Tian J, Pu H, Yusufu A, Cao L
PloS one 2017;12(3):e0172499
PloS one 2017;12(3):e0172499
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Mouse Angiopoietin-1 by Western Blot. Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Angiopoietin-1 Monoclonal Antibody (Catalog # MAB8220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
