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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- AP07808SU-N - Provider product page
- Provider
- OriGene
- Product name
- Angiopoietin 1 (ANGPT1) (N-term) rabbit polyclonal antibody, Serum
- Antibody type
- Polyclonal
- Description
- Angiopoietin 1 (ANGPT1) (N-term) rabbit polyclonal antibody, Serum
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ANGPT1
- Antibody clone number
- NULL
- Vial size
- 50 µl
- Concentration
- 80 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot against supernatants of Mouse Angiopoietin-expressing endothelial cells using Angiopoietin-1 antibody at a 1/500 dilution to detect Mouse Ang-1. Lane 1: Wt endothelial cells. Lane 2: Mouse Ang-1 (clone 1-8) expressing cells. Lane 3: Mouse Ang-1 (clone 1-15) expressing cells. Lane 4: Mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 ug of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1/5000 dilution of HRP conjugated Goat anti Rabbit IgG preceded color development.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded Mouse Liver stained with Angiopoietin-1 antibody after heat-induced antigen retrieval.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded Mouse Kidney stained with Angiopoietin-1 antibody after heat-induced antigen retrieval.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded Human Lung Vessel stained with Angiopoietin-1 antibody after heat-induced antigen retrieval.
- Validation comment
- IHC
