Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C744593 - Provider product page
- Provider
- LSBio
- Product name
- ANGPT1 / Angiopoietin-1 Antibody (aa15-45) LS-C744593
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit anti-ANG1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.