PA1-32150

antibody from Invitrogen Antibodies

Targeting: ANGPT1 Ang1, KIAA0003

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-32150

Provider

Invitrogen Antibodies

Product name

Angiopoietin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is specific for mouse Angiopoietin 1 (mAng-1). Partial cross-reactivity is noted with human Angiopoietin 2. Store as a concentrated solution. Centrifuge briefly prior to opening vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

80 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Efficiency of Gymnema sylvestre-derived gymnemic acid on the restoration and improvement of brain vascular characteristics in diabetic rats.

Sandech N, Jangchart R, Komolkriengkrai M, Boonyoung P, Khimmaktong W
Experimental and therapeutic medicine 2021 Dec;22(6):1420

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Angiopoietin 1 was performed by separating 20 µg of various supernatants of mouse angiopoietin-expressing endothelial cells by 10% SDS-PAGE. Angiopoietin 1 Polyclonal Antibody (Product # PA1-32150) was used as the primary antibody. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 11 Densitometric analysis of VEGF and Ang-1 protein expression in the hypothalamus of control, CGM, STZ, STZ + GM, and STZ + GL rats at 8 weeks. Data are presented as the mean +- SEM. * P