R1585

antibody from Acris Antibodies GmbH

Targeting: ANGPT1 Ang1, KIAA0003

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1585

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1585, RRID:AB_972866

Product name

anti Angiopoietin-1

Antibody type

Polyclonal

Antigen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a region (aa 21-40) near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.2 ml

Concentration

85 mg/ml (by Refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoblotting. Rabbit anti-Ang 1 antibody was used at a 1/500 dilution to detect Mouse Ang-1 by western blot found in supernatants of Mouse-angiopoietin-expressing endothelial cells. Lane 1: wt endothelial cells. Lane 2: Mouse Ang-1 (clone 1-8) expressing cells. Lane 3: Mouse Ang-1 (clone 1-15) expressing cells. Lane 4: Mouse Ang-2 (clone 2-9) expressing cells.Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Immunoprecipitation preceded reaction with primary antibody at RT for 1 h. After subsequent washing, a 1/5,000 dilution of HRP conjugated Goat-anti Rabbit IgG (R1454HRP) preceded color development.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Anti-ANG1 antibody was diluted 1/500 to detect ANG1 in Formalin Fixed and Paraffin Embedded Human lung tissue. Tissue was . No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.