LS-C185971

antibody from LSBio

Targeting: H2AZ1 H2A.Z, H2AFZ, H2AZ

Western blot (Enhanced validation)

Immunocytochemistry (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185971

Provider

LSBio

Product name

H2AFZ / H2A.z Antibody LS-C185971

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: JurKat. 15% SDS PAGE. H2AFZ / H2A.z antibody diluted at 1:1000.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431 using Histone H2A.Z antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431 using Histone H2A.Z antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded SCC4 xenograft using Histone H2A.Z antibody at 1:100 dilution.