PA5-112439

antibody from Invitrogen Antibodies

Targeting: H2AZ1 H2A.Z, H2AFZ, H2AZ

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112439

Provider

Invitrogen Antibodies

Product name

H2A.ZK11ac Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.12 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Acetyl-H2A.ZK11ac (Lys11) in Hela cells using a Acetyl-H2A.ZK11ac (Lys11) polyclonal antibody (Product # PA5-112439) at a dilution of 1:100. The cells were treated with 30mM sodium butyrate for 4 hours. The cells were then fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation analysis of Acetyl-H2A.ZK11ac (Lys11) in 4*10^6 Hela cells using a Acetyl-H2A.ZK11ac (Lys11) polyclonal antibody (Product #PA5-112439). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8 µg anti-H2AFZ or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.