Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-22268 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PHAX Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Gebhardt A, Habjan M, Benda C, Meiler A, Haas DA, Hein MY, Mann A, Mann M, Habermann B, Pichlmair A
Nature communications 2015 Sep 18;6:8192
Nature communications 2015 Sep 18;6:8192
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody NBP2-22268 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. NBP2-22268 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PHAX Antibody [NBP2-22268] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22268 used for IP at 6 ug/mg lysate. PHAX was also immunoprecipitated by rabbit anti-PHAX antibody. For blotting immunoprecipitated PHAX, NBP2-22268 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.