20459-1-AP

antibody from Proteintech Group

Targeting: SLC39A8 BIGM103

Provider product page for 20459-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [39]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20459-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20459-1-AP, RRID:AB_10697830
Product name: ZIP8 antibody
Antibody type: Polyclonal
Description: KD/KO validated ZIP8 antibody (Cat. #20459-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SLC39A8 protein structure - 20459-1-AP shown in red.

Submitted references Intracellular Zn(2+) promotes extracellular matrix remodeling in dexamethasone-treated trabecular meshwork.

Liu C, Tang J, Chen Y, Zhang Q, Lin J, Wu S, Han J, Liu Z, Wu C, Zhuo Y, Li Y
American journal of physiology. Cell physiology 2024 May 1;326(5):C1293-C1307

Integrating Transcriptomics and Proteomics to Characterize the Intestinal Responses to Cadmium Exposure Using a Piglet Model.

Li Y, Pan Y, Yin Y, Huang R
International journal of molecular sciences 2024 Jun 12;25(12)

Identification of ZIP8-induced ferroptosis as a major type of cell death in monocytes under sepsis conditions.

Zhang T, Wang S, Hua D, Shi X, Deng H, Jin S, Lv X
Redox biology 2024 Feb;69:102985

Effects of Zinc Combined with Metformin on Zinc Homeostasis, Blood-Epididymal Barrier, and Epididymal Absorption in Male Diabetic Mice.

Zhang M, Li H, Ma J, Yang C, Yang Y, Zhao B, Tie Y, Wang S
Biological trace element research 2024 Apr 8;

circAMN1-Mediated Ferroptosis Regulates the Expulsion of Placenta in Trophoblast Cells.

Lv C, Guo L, Wang Y, Li Z, Zhao X, Zhang Y
Antioxidants (Basel, Switzerland) 2024 Apr 11;13(4)

Hepatobiliary manganese homeostasis is dynamic in the setting of inflammation or infection in mice.

Sunuwar L, Tomar V, Wildeman A, Culotta V, Melia J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Sep;37(9):e23123

Hepatobiliary manganese homeostasis is dynamic in the setting of illness in mice.

Sunuwar L, Tomar V, Wildeman A, Culotta V, Melia J
bioRxiv : the preprint server for biology 2023 Mar 24;

Proton-Pump Inhibitors Suppress T Cell Response by Shifting Intracellular Zinc Distribution.

Liu W, Jakobs J, Rink L
International journal of molecular sciences 2023 Jan 7;24(2)

Ferroptosis is involved in corpus cavernosum smooth muscle cells impairment in diabetes mellitus-induced erectile dysfunction.

Xu W, Sun T, Wang J, Wang T, Wang S, Liu J, Liu K, Li H
Andrology 2023 Feb;11(2):332-343

Protective effects of ZIP8 on Toxoplasma gondii-induced acute hepatocyte injury in mice.

Wang Y, Wang C, Chen H, Zhang Y, Gao N, Yu Y, Xing Y, Xie L, Wang Z, Cai Y
Acta tropica 2022 Oct;234:106629

Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction.

Henning Y, Blind US, Larafa S, Matschke J, Fandrey J
Cell death & disease 2022 Jul 29;13(7):662

An Iron Metabolism-Related Gene Signature for the Prognosis of Colon Cancer.

Yuan J, Liu T, Zhang Y
Frontiers in cell and developmental biology 2021;9:786684

Expression profiles of SLC39A/ZIP7, ZIP8 and ZIP14 in response to exercise-induced skeletal muscle damage.

Liu J, Xu C, Yu X, Zuo Q
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2021 Sep;67:126784

ZIP8 mediates the extracellular matrix degradation of nucleus pulposus cells via NF-κB signaling pathway.

Ding W, Ge Y, Sun H, Xu J, Gu H, Bian C, Chen H, Jiang L, Yin X
Biochemical and biophysical research communications 2021 Apr 23;550:30-36

Localization of ZIP14 and ZIP8 in HIBCPP Cells.

Morgan SE, Schroten H, Ishikawa H, Zhao N
Brain sciences 2020 Aug 8;10(8)

Segment-specific and direction-dependent transport of cadmium and manganese in immortalized S1, S2, and S3 cells derived from mouse kidney proximal tubules.

Fujishiro H, Hamao S, Isawa M, Himeno S
The Journal of toxicological sciences 2019;44(9):611-619

The acute phase serum zinc concentration is a reliable biomarker for predicting the functional outcome after spinal cord injury.

Kijima K, Kubota K, Hara M, Kobayakawa K, Yokota K, Saito T, Yoshizaki S, Maeda T, Konno D, Matsumoto Y, Nakashima Y, Okada S
EBioMedicine 2019 Mar;41:659-669

A sodium-glucose cotransporter 2 (SGLT2) inhibitor dapagliflozin comparison with insulin shows important effects on Zn(2+)-transporters in cardiomyocytes from insulin-resistant metabolic syndrome rats through inhibition of oxidative stress (1).

Olgar Y, Turan B
Canadian journal of physiology and pharmacology 2019 Jun;97(6):528-535

Manganese Uptake by A549 Cells is Mediated by Both ZIP8 and ZIP14.

Scheiber IF, Alarcon NO, Zhao N
Nutrients 2019 Jun 28;11(7)

Induction of the metal transporter ZIP8 by interferon gamma in intestinal epithelial cells: Potential role of metal dyshomeostasis in Crohn's disease.

Melia JMP, Lin R, Xavier RJ, Thompson RB, Fu D, Wan F, Sears CL, Donowitz M
Biochemical and biophysical research communications 2019 Jul 23;515(2):325-331

Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn(2+) Levels with Re-Distribution of Zn(2+)-Transporters in Aged Rat Cardiomyocytes.

Olgar Y, Tuncay E, Turan B
International journal of molecular sciences 2019 Aug 2;20(15)

ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx.

Cheng G, Chang FJ, You PH, Lin J, Huang XY, Wu HY, Yan L, Deng JZ, You HJ, Sun CF
International immunopharmacology 2018 Sep;62:203-211

A Faroese founder variant in TBCD causes early onset, progressive encephalopathy with a homogenous clinical course.

Grønborg S, Risom L, Ek J, Larsen KB, Scheie D, Petkov Y, Larsen VA, Dunø M, Joensen F, Østergaard E
European journal of human genetics : EJHG 2018 Oct;26(10):1512-1520

Induction of endoplasmic reticulum stress and changes in expression levels of Zn(2+)-transporters in hypertrophic rat heart.

Olgar Y, Ozdemir S, Turan B
Molecular and cellular biochemistry 2018 Mar;440(1-2):209-219

Increased free Zn(2+) correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn(2+) -transporters in heart failure.

Olgar Y, Durak A, Tuncay E, Bitirim CV, Ozcinar E, Inan MB, Tokcaer-Keskin Z, Akcali KC, Akar AR, Turan B
Journal of cellular and molecular medicine 2018 Mar;22(3):1944-1956

Tubular iron deposition and iron handling proteins in human healthy kidney and chronic kidney disease.

van Raaij S, van Swelm R, Bouman K, Cliteur M, van den Heuvel MC, Pertijs J, Patel D, Bass P, van Goor H, Unwin R, Srai SK, Swinkels D
Scientific reports 2018 Jun 19;8(1):9353

Role of ZIP8 in regulating cell morphology and NF-κB/Snail2 signaling.

Geng X, Liu L, Banes-Berceli A, Yang Z, Kang P, Shen J, Tsai KJ, Liu Z
Metallomics : integrated biometal science 2018 Jul 18;10(7):953-964

Concentration-dependent roles of DMT1 and ZIP14 in cadmium absorption in Caco-2 cells.

Fujishiro H, Hamao S, Tanaka R, Kambe T, Himeno S
The Journal of toxicological sciences 2017;42(5):559-567

Inhibitory effect of divalent metal cations on zinc uptake via mouse Zrt-/Irt-like protein 8 (ZIP8).

Koike A, Sou J, Ohishi A, Nishida K, Nagasawa K
Life sciences 2017 Mar 15;173:80-85

Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.

Lin W, Vann DR, Doulias PT, Wang T, Landesberg G, Li X, Ricciotti E, Scalia R, He M, Hand NJ, Rader DJ
The Journal of clinical investigation 2017 Jun 1;127(6):2407-2417

Analysis of ZIP (Zrt-, Irt-related protein) transporter gene expression in murine neural stem/progenitor cells.

Nishikawa M, Mori H, Hara M
Environmental toxicology and pharmacology 2017 Jul;53:81-88

Effects of developmental exposure to manganese and/or low iron diet: Changes to metal transporters, sucrose preference, elevated zero-maze, open-field, and locomotion in response to fenfluramine, amphetamine, and MK-801.

Amos-Kroohs RM, Bloor CP, Qureshi MA, Vorhees CV, Williams MT
Toxicology reports 2015;2:1046-1056

Decreased Zn(2+) Influx Underlies the Protective Role of Hypoxia in Rat Nucleus Pulposus Cells.

Yin XF, Jiang LB, Ma YQ, Xu J, Gu HJ, Wu XH, Li XL, Dong J
Biological trace element research 2015 Nov;168(1):196-205

Zinc deficiency mediates alcohol-induced apoptotic cell death in the liver of rats through activating ER and mitochondrial cell death pathways.

Sun Q, Zhong W, Zhang W, Li Q, Sun X, Tan X, Sun X, Dong D, Zhou Z
American journal of physiology. Gastrointestinal and liver physiology 2015 May 1;308(9):G757-66

Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.

Grubman A, Lidgerwood GE, Duncan C, Bica L, Tan JL, Parker SJ, Caragounis A, Meyerowitz J, Volitakis I, Moujalled D, Liddell JR, Hickey JL, Horne M, Longmuir S, Koistinaho J, Donnelly PS, Crouch PJ, Tammen I, White AR, Kanninen KM
Acta neuropathologica communications 2014 Feb 28;2:25

Regulation of the catabolic cascade in osteoarthritis by the zinc-ZIP8-MTF1 axis.

Kim JH, Jeon J, Shin M, Won Y, Lee M, Kwak JS, Lee G, Rhee J, Ryu JH, Chun CH, Chun JS
Cell 2014 Feb 13;156(4):730-43

Interleukin-6 enhances manganese accumulation in SH-SY5Y cells: implications of the up-regulation of ZIP14 and the down-regulation of ZnT10.

Fujishiro H, Yoshida M, Nakano Y, Himeno S
Metallomics : integrated biometal science 2014 Apr;6(4):944-9

Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses?

Grubman A, Pollari E, Duncan C, Caragounis A, Blom T, Volitakis I, Wong A, Cooper J, Crouch PJ, Koistinaho J, Jalanko A, White AR, Kanninen KM
Metallomics : integrated biometal science 2014 Apr;6(4):932-43

Altered biometal homeostasis is associated with CLN6 mRNA loss in mouse neuronal ceroid lipofuscinosis.

Kanninen KM, Grubman A, Caragounis A, Duncan C, Parker SJ, Lidgerwood GE, Volitakis I, Ganio G, Crouch PJ, White AR
Biology open 2013 Jun 15;2(6):635-46

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse lung tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP(SLC39A8 antibody) at dilution of 1:800
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using SLC39A8 antibody 20459-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human placenta using 20459-1-AP(SLC39A8 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human placenta using 20459-1-AP(SLC39A8 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue