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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-111913 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC39A8 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC39A8 was performed in human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates. Lane 1,2: SLC39A8 (extracellular) Antibody (Product # PA5-111913) at a dilution of 1:200. Lane 3,4: SLC39A8 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC39A8 was performed in mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates. Lane 1,2: SLC39A8 (extracellular) Antibody (Product # PA5-111913) at a dilution of 1:200. Lane 3,4: SLC39A8 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC39A8 was performed in human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 4) lysates. Lane 1,2: SLC39A8 (extracellular) Antibody (Product # PA5-111913) at a dilution of 1:200. Lane 3,4: SLC39A8 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC39A8 was performed in rat brain lysate. Lane 1: SLC39A8 (extracellular) Antibody (Product # PA5-111913) at a dilution of 1:200. Lane 2: SLC39A8 (extracellular) Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC39A8 was performed in mouse kidney (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates. Lane 1,2: SLC39A8 (extracellular) Antibody (Product # PA5-111913) at a dilution of 1:200. Lane 3,4: SLC39A8 (extracellular) Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC39A8 in perfusion-fixed, frozen rat hippocampus tissue sections using SLC39A8 (extracellular) Antibody (Product # PA5-111913) at a dilution of 1:200, followed by goat-anti-rabbit-AlexaFluor-488. ZIP8 staining (green) appears in interneurons (arrows) adjacent to the granular layer (GL). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of SLC39A8 in live intact human THP-1 acute monocytic leukemia cells. Black: Cells. Pink: Cells and goat-anti-rabbit-FITC. Green: Cells, SLC39A8 (extracellular) Antibody (Product # PA5-111913), and goat-anti-rabbit-FITC.
