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- Product number
- PA5-26368 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC39A8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis.
Liang J, Huang G, Liu X, Taghavifar F, Liu N, Wang Y, Deng N, Yao C, Xie T, Kulur V, Dai K, Burman A, Rowan SC, Weigt SS, Belperio J, Stripp B, Parks WC, Jiang D, Noble PW
The Journal of clinical investigation 2022 Jun 1;132(11)
The Journal of clinical investigation 2022 Jun 1;132(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using a S39A8 polyclonal antibody (Product # PA5-26368) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 ZIP8-dependent zinc metabolism is required for AEC2 renewal. ( A ) Representative plots of intracellular zinc in gated healthy and IPF AEC2s by flow cytometry. ( B ) Percentage of zinc-positive AEC2s within the gated AEC2 population from healthy ( n = 5) and IPF lungs ( n = 11) (** P < 0.01). ( C ) Flow cytometry plots of gated ZIP8 + and ZIP8 - AEC2s. ( D ) CFE of flow-sorted ZIP8 + and ZIP8 - AEC2s from healthy ( n = 6) and IPF lungs ( n = 4) (*** P < 0.001, **** P < 0.0001). ( E ) PDPN expression in AEC2s derived from 3D-cultured organoids ( n = 3, * P < 0.05). ( F ) CFE of AEC2s from healthy and IPF lungs with and without ZnSO 4 (100 muM) treatment ( n = 5-7, **** P < 0.0001). ( G ) CFE of ZIP8 + and ZIP8 - AEC2s with and without ZnSO 4 (100 muM) treatment ( n = 3 each, **** P < 0.0001). ( H ) CFE of AEC2s with and without ZnSO 4 and TPEN (1 muM) treatment ( n = 3-5, ** P < 0.01, **** P < 0.0001 by ANOVA). ( I and J ) Expression of SLC39A8 ( n = 4-6, **** P < 0.0001) and SFTPC ( n = 4-6, *** P < 0.001) in AEC2s with and without ZnSO 4 treatment by qPCR. Data are shown as mean +- SEM. B and E : unpaired 2-tailed Student's t test; D , F , G , H , I , and J : 2-way ANOVA.
