AF1678

antibody from Novus Biologicals

Targeting: FABP3 FABP11, H-FABP, MDGI, O-FABP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1678

Provider

Novus Biologicals

Product name

Sheep Polyclonal FABP3/H-FABP Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FABP3/H-FABP in direct ELISAs and detects human, mouse, and rat FABP3/H-FABP in Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) FABP4 and recombinant human (rh) FABP4 is observed, approximately 15% cross-reactivity with recombinant rat (rr) FABP1, rhFABP1, rhFABP2, rhFABP7, and rhFABP8 is observed, and less than 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.

Reactivity

Human, Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.

Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
Hippocampus 2011 Feb;21(2):162-71

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Formation of a human-derived fat tissue layer in P(DL)LGA hollow fibre scaffolds for adipocyte tissue engineering.

Morgan SM, Ainsworth BJ, Kanczler JM, Babister JC, Chaudhuri JB, Oreffo RO
Biomaterials 2009 Apr;30(10):1910-7

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The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells.

Xu Y, Mirmalek-Sani SH, Yang X, Zhang J, Oreffo RO
Experimental cell research 2006 Jun 10;312(10):1856-64

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat FABP3/H-FABP by Western Blot. Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse FABP3/H-FABP by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

FABP3/H-FABP in Human Heart. FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.