Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Blocking/Neutralizing [2]
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Validation data
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- Product number
- LS-C407972 - Provider product page
- Provider
- LSBio
- Product name
- FABP3 / H-FABP Antibody (aa15-48) LS-C407972
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cardiac FABP antibody Western blot. All lanes: Anti Cardiac FABP at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Predicted band size: 20 kD. Observed band size: 20 kD.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cardiac FABP antibody IHC-paraffin. IHC(P): Mouse Cardiac Muscle Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cardiac FABP antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cardiac FABP antibody IHC-paraffin. IHC(P): Mouse Cardiac Muscle Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cardiac FABP antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysate, Lane 2: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cardiac FABP antibody Western blot. All lanes: Anti Cardiac FABP at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Predicted band size: 20 kD. Observed band size: 20 kD.