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LearnPA5-27144
antibody from Invitrogen Antibodies
Targeting: FOXM1
FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP2, TGT3, trident
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-27144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: FOXM1-transfected 293T.
- Concentration
- 1.52 mg/mL
Submitted references Generation of a conditional mutant knock-in under the control of the natural promoter using CRISPR-Cas9 and Cre-Lox systems.
Thakur VS, Welford SM
PloS one 2020;15(10):e0240256
PloS one 2020;15(10):e0240256
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXM1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human FOXM1-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FOXM1 polyclonal antibody (Product # PA5-27144) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FOXM1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FOXM1 Polyclonal Antibody (Product # PA5-27144) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 6 Effect of SAT1 mutation on its downstream targets genes. A. Western blot of parental U87MG and knockin clones DM#2 and SM#36 demonstrating insignificant changes to basal levels of FOXM1, MELK, and SAT1. B. Quantification of three replicate experiments. C. Western blot of Cre infected DM#2 and SM#36 showing changes in FOXM1 and MELK. D. Quantification of three replicate experiments. Uncropped and replicate westerns are shown in supplementary data.
