Explore
Validate
LearnPA5-27622
antibody from Invitrogen Antibodies
Targeting: FOXM1
FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP2, TGT3, trident
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27622 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human FOXM1-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.72 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXM1 using A) 30 µg 293T whole cell lysate and B) 30 µg A431 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FOXM1 polyclonal antibody (Product # PA5-27622) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXM1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human FOXM1-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FOXM1 polyclonal antibody (Product # PA5-27622) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FOXM1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FOXM1 Polyclonal Antibody (Product # PA5-27622) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FOXM1 Polyclonal Antibody detects FOXM1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. FOXM1 stained by FOXM1 Polyclonal Antibody (Product # PA5-27622) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
