Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- AF4496 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal EDNRB/Endothelin R Type B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EDNRB in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human EDNRB/Endothelin R Type B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, A172 human glioblastoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EDNRB/Endothelin R Type B at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- EDNRB/Endothelin R Type B in A172 Human Cell Line. EDNRB/Endothelin R Type B was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry. Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry.