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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunocytochemistry [1]
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- Product number
- M805 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGF Monoclonal Antibody (1H11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- The M805 anti-EGF antibody (Clone 1H11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M806B (biotinylated conjugate of Clone 3A8). Typical dilutions for sandwich ELISA include 1-3 µgs/mL for coating and 0.125-0.5 µg/mL for detection.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1H11
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references β-Hexachlorocyclohexane Drives Carcinogenesis in the Human Normal Bronchial Epithelium Cell Line BEAS-2B.
Rubini E, Minacori M, Paglia G, Altieri F, Chichiarelli S, Romaniello D, Eufemi M
International journal of molecular sciences 2021 May 29;22(11)
International journal of molecular sciences 2021 May 29;22(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EGF (green) in HeLa cells either left untreated (left panel) or treated with 10 ng/mL EGF (right panel) for 5 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an EGF monoclonal antibody (Product # M805) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of Human EGF was performed using the Thermo Scientific ELISA Kit (Product # EHEGF) by coating a blank 96-well microtiter plate with 100 µL per well of Human EGF moncolonal antibody (Product # M805) in duplicate at 1, 3, 4, 5, 7, and 9 µg/mL in DPBS (Product # 28374) and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300 µL per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human EGF recombinant protein at 50 µL per well was added in duplicate at 500, 250, 125, 62.5, 31.25, 15.625, 7.8125 and 0 pg/mL for 2 hours at room temperature along with 50 µL of Human EGF biotinylated monoclonal antibody (Product # M806B) in all applicable wells at 0.2 µg/mL for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer (Product # N503) and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:130,000 dilution for 1 hour at room temperature and then washed and incubated with 100 µL per well of TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.
