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- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB5256 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal EGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Pro EGF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Amphiregulin, rmEpigen, rmEpiregulin, rmEGF, rmMFG-E8, recombinant human (rh) EGF, rhHB-EGF, rhHRG alpha, rhHRG beta, or recombinant rat EGF is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Pro EGF in Mouse Kidney. Pro Epidermal Growth Factor (Pro EGF) was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Pro EGF Monoclonal Antibody (Catalog # MAB5256) at 10 µg/mL for 3 hours at room temperature. Tissue was stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
