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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- 701538 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGF Recombinant Rabbit Monoclonal Antibody (3H11L6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3H11L6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Localisation of epidermal growth factor (EGF), its specific receptor (EGF-R) and aromatase at the materno-fetal interface during placentation in the pregnant mare.
Allen WR, Gower S, Wilsher S
Placenta 2017 Feb;50:53-59
Placenta 2017 Feb;50:53-59
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis was performed using EGF Recombinant Rabbit Monoclonal Antibody (Product # 701538) at 2 µg/mL. A ten point standard curve was plotted with full length active recombinant EGF protein as a standard with concentrations ranging from 0.3 pg/mL to 12.5 ng/mL. An Anti-EGF antibody conjugated to biotin was used as a detector at a concentration of 2 µg/mL. A non-linear regression analysis (4 PL) was performed and LOD and LOQ for the antibody pair was determined.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Human PBMCs stained with EGF Recombinant Rabbit Monoclonal Antibody (Product # 701538) using Alexa Fluor® 488 Goat anti-Rabbit as a secondary antibody ("a", green) and DAPI to stain the nuclei ("b", blue). A composite image of cells showing nuclear and epidermal localization of EGF is also shown ("c").
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis was performed using EGF Recombinant Rabbit Monoclonal Antibody (Product # 701538) at 2 æg/mL. A ten point standard curve was plotted with full length active recombinant EGF protein as a standard with concentrations ranging from 0.3 pg/mL to 12.5 ng/mL. An Anti-EGF antibody conjugated to biotin was used as a detector at a concentration of 2 æg/mL. A non-linear regression analysis (4 PL) was performed and LOD and LOQ for the antibody pair was determined.
