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- Antibody Data
- Antigen structure
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- Product number
- 710569 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGF Recombinant Polyclonal Antibody (3 HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3 HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA analysis using a ten point standard curve plotted with a full-length active recombinant EGF protein standard at concentrations ranging from 12.5 ng/mL to 0.3 pg/mL bound by Anti-EGF capture antibody coated onto the plate. The standard was detected using a biotin-conjugated EGF Recombinant Rabbit Polyclonal Antibody (Product # 710569) at a concentration of 2 µg/mL. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody pair was determined.
