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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29229 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- pro-EGF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Predicted reactivity: Rat (80%), Dog (85%), Cat (86%), Pig (85%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of pro-EGF was performed by separating 50 µg of rat tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a pro-EGF Polyclonal Antibody (Product # PA5-29229) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGF (Product # PA5-29229) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
