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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-236-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human EGF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat EGF and recombinant mouse EGF is observed. Does not neutralize the biological activity of recombinant human TGF-alpha .
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01133- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Human EGF Polyclonal Antibody (Catalog # AB-236-NA). The ND50 is typically 0.08 -0.8 µg/mL.
