Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF236 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat EGF and 5% cross-reactivity with recombinant mouse EGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Pakuła M, Mikuła-Pietrasik J, Witucka A, Kostka-Jeziorny K, Uruski P, Moszyński R, Naumowicz E, Sajdak S, Tykarski A, Książek K
International journal of molecular sciences 2019 Jan 2;20(1)
International journal of molecular sciences 2019 Jan 2;20(1)
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
American journal of respiratory cell and molecular biology 2007 Nov;37(5):578-88
American journal of respiratory cell and molecular biology 2007 Nov;37(5):578-88
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
Biology of reproduction 2007 Jul;77(1):53-60
Biology of reproduction 2007 Jul;77(1):53-60
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72
Investigative ophthalmology & visual science 2006 Mar;47(3):864-72
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Developmental biology 2004 Feb 15;266(2):223-37
Developmental biology 2004 Feb 15;266(2):223-37
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Developmental biology 2004 Feb 15;266(2):223-37
Developmental biology 2004 Feb 15;266(2):223-37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human, Mouse, and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EGF in Human Placenta. EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND50 is typically 0.04-0.08 µg/mL.