16006-1-AP

antibody from Proteintech Group

Targeting: CISD1 C10orf70, MDS029, mitoNEET, ZCD1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

16006-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16006-1-AP, RRID:AB_2080268

Product name

CISD1 antibody

Antibody type

Polyclonal

Description

KD/KO validated CISD1 antibody (Cat. #16006-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Inhibition of CISD1 attenuates cisplatin-induced hearing loss in mice via the PI3K and MAPK pathways.

Dong W, Jiang Y, Yao Q, Xu M, Jin Y, Dong L, Li Z, Yu D
Biochemical pharmacology 2024 May;223:116132

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Mitochondrial GCN5L1 acts as a novel regulator for iron homeostasis to promote sorafenib sensitivity in hepatocellular carcinoma.

Hu X, Zhang P, Li S, Zhang J, Wang D, Wang Z, Zhu L, Wang L
Journal of translational medicine 2024 Jun 25;22(1):593

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Mitochondrial CISD1/Cisd accumulation blocks mitophagy and genetic or pharmacological inhibition rescues neurodegenerative phenotypes in Pink1/parkin models.

Martinez A, Sanchez-Martinez A, Pickering JT, Twyning MJ, Terriente-Felix A, Chen PL, Chen CH, Whitworth AJ
Molecular neurodegeneration 2024 Jan 25;19(1):12

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Nix interacts with WIPI2 to induce mitophagy.

Bunker EN, Le Guerroué F, Wang C, Strub MP, Werner A, Tjandra N, Youle RJ
The EMBO journal 2023 Nov 15;42(22):e113491

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Elucidation of ubiquitin-conjugating enzymes that interact with RBR-type ubiquitin ligases using a liquid-liquid phase separation-based method.

Hayashida R, Kikuchi R, Imai K, Kojima W, Yamada T, Iijima M, Sesaki H, Tanaka K, Matsuda N, Yamano K
The Journal of biological chemistry 2023 Feb;299(2):102822

---

FDX1 regulates cellular protein lipoylation through direct binding to LIAS.

Dreishpoon MB, Bick NR, Petrova B, Warui DM, Cameron A, Booker SJ, Kanarek N, Golub TR, Tsvetkov P
bioRxiv : the preprint server for biology 2023 Feb 4;

---

GeneMAP: A discovery platform for metabolic gene function.

Khan A, Unlu G, Lin P, Liu Y, Kilic E, Kenny TC, Birsoy K, Gamazon ER
bioRxiv : the preprint server for biology 2023 Dec 8;

---

PINK1 and Parkin regulate IP(3)R-mediated ER calcium release.

Ham SJ, Yoo H, Woo D, Lee DH, Park KS, Chung J
Nature communications 2023 Aug 25;14(1):5202

---

Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stress.

Lee S, Seok BG, Lee SJ, Chung SW
Cell death & disease 2022 Feb 8;13(2):127

---

Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.

Li L, Yu S, Hu Q, Hai Y, Li Y
International journal of biological sciences 2021;17(12):3133-3144

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Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Antico O, Ordureau A, Stevens M, Singh F, Nirujogi RS, Gierlinski M, Barini E, Rickwood ML, Prescott A, Toth R, Ganley IG, Harper JW, Muqit MMK
Science advances 2021 Nov 12;7(46):eabj0722

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PPARγ activation improves the microenvironment of perivascular adipose tissue and attenuates aortic stiffening in obesity.

Chen JY, Wu YP, Li CY, Jheng HF, Kao LZ, Yang CC, Leu SY, Lien IC, Weng WT, Tai HC, Chiou YW, Tang MJ, Tsai PJ, Tsai YS
Journal of biomedical science 2021 Mar 29;28(1):22

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AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy.

Hung CM, Lombardo PS, Malik N, Brun SN, Hellberg K, Van Nostrand JL, Garcia D, Baumgart J, Diffenderfer K, Asara JM, Shaw RJ
Science advances 2021 Apr;7(15)

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A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.

Watanabe M, Saeki Y, Takahashi H, Ohtake F, Yoshida Y, Kasuga Y, Kondo T, Yaguchi H, Suzuki M, Ishida H, Tanaka K, Hatakeyama S
Communications biology 2020 Oct 20;3(1):592

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Bioenergetic restoration and neuroprotection after therapeutic targeting of mitoNEET: New mechanism of pioglitazone following traumatic brain injury.

Yonutas HM, Hubbard WB, Pandya JD, Vekaria HJ, Geldenhuys WJ, Sullivan PG
Experimental neurology 2020 May;327:113243

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Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling.

Ordureau A, Paulo JA, Zhang J, An H, Swatek KN, Cannon JR, Wan Q, Komander D, Harper JW
Molecular cell 2020 Mar 5;77(5):1124-1142.e10

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Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.

Molino D, Pila-Castellanos I, Marjault HB, Dias Amoedo N, Kopp K, Rochin L, Karmi O, Sohn YS, Lines L, Hamaï A, Joly S, Radreau P, Vonderscher J, Codogno P, Giordano F, Machin P, Rossignol R, Meldrum E, Arnoult D, Ruggieri A, Nechushtai R, de Chassey B, Morel E
EMBO reports 2020 Dec 3;21(12):e49019

---

Translocase of the outer mitochondrial membrane complex subunit 20 (TOMM20) facilitates cancer aggressiveness and therapeutic resistance in chondrosarcoma.

Roche ME, Lin Z, Whitaker-Menezes D, Zhan T, Szuhai K, Bovee JVMG, Abraham JA, Jiang W, Martinez-Outschoorn U, Basu-Mallick A
Biochimica et biophysica acta. Molecular basis of disease 2020 Dec 1;1866(12):165962

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Crystal structure of the mitochondrial protein mitoNEET bound to a benze-sulfonide ligand.

Geldenhuys WJ, Long TE, Saralkar P, Iwasaki T, Nuñez RAA, Nair RR, Konkle ME, Menze MA, Pinti MV, Hollander JM, Hazlehurst LA, Robart AR
Communications chemistry 2019;2

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Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy.

Heo JM, Harper NJ, Paulo JA, Li M, Xu Q, Coughlin M, Elledge SJ, Harper JW
Science advances 2019 Nov;5(11):eaay4624

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Parkin recruitment to impaired mitochondria for nonselective ubiquitylation is facilitated by MITOL.

Koyano F, Yamano K, Kosako H, Tanaka K, Matsuda N
The Journal of biological chemistry 2019 Jun 28;294(26):10300-10314

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Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes.

Koyano F, Yamano K, Kosako H, Kimura Y, Kimura M, Fujiki Y, Tanaka K, Matsuda N
EMBO reports 2019 Dec 5;20(12):e47728

---

Quantitative Proteomics of Presynaptic Mitochondria Reveal an Overexpression and Biological Relevance of Neuronal MitoNEET in Postnatal Brain Development.

Stauch KL, Villeneuve LM, Totusek S, Lamberty B, Ciborowski P, Fox HS
Developmental neurobiology 2019 Apr;79(4):370-386

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RAB7A phosphorylation by TBK1 promotes mitophagy via the PINK-PARKIN pathway.

Heo JM, Ordureau A, Swarup S, Paulo JA, Shen K, Sabatini DM, Harper JW
Science advances 2018 Nov;4(11):eaav0443

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Phosphorylation of Parkin at serine 65 is essential for its activation in vivo.

McWilliams TG, Barini E, Pohjolan-Pirhonen R, Brooks SP, Singh F, Burel S, Balk K, Kumar A, Montava-Garriga L, Prescott AR, Hassoun SM, Mouton-Liger F, Ball G, Hills R, Knebel A, Ulusoy A, Di Monte DA, Tamjar J, Antico O, Fears K, Smith L, Brambilla R, Palin E, Valori M, Eerola-Rautio J, Tienari P, Corti O, Dunnett SB, Ganley IG, Suomalainen A, Muqit MMK
Open biology 2018 Nov 7;8(11)

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Hydrogen Sulfide Is Increased in Oral Squamous Cell Carcinoma Compared to Adjacent Benign Oral Mucosae.

Meram AT, Chen J, Patel S, Kim DD, Shirley B, Covello P, Coppola D, Wei EX, Ghali G, Kevil CG, Shackelford RE
Anticancer research 2018 Jul;38(7):3843-3852

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Basal Mitophagy Occurs Independently of PINK1 in Mouse Tissues of High Metabolic Demand.

McWilliams TG, Prescott AR, Montava-Garriga L, Ball G, Singh F, Barini E, Muqit MMK, Brooks SP, Ganley IG
Cell metabolism 2018 Feb 6;27(2):439-449.e5

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Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics.

Ordureau A, Paulo JA, Zhang W, Ahfeldt T, Zhang J, Cohn EF, Hou Z, Heo JM, Rubin LL, Sidhu SS, Gygi SP, Harper JW
Molecular cell 2018 Apr 19;70(2):211-227.e8

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Mechanism and regulation of the Lys6-selective deubiquitinase USP30.

Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
Nature structural & molecular biology 2017 Nov;24(11):920-930

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Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe(-/-) mice.

Heritage M, Jaskowski L, Bridle K, Campbell C, Briskey D, Britton L, Fletcher L, Vitetta L, Subramaniam VN, Crawford D
World journal of gastrointestinal pathophysiology 2017 May 15;8(2):67-76

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MitoNEET (CISD1) Knockout Mice Show Signs of Striatal Mitochondrial Dysfunction and a Parkinson's Disease Phenotype.

Geldenhuys WJ, Benkovic SA, Lin L, Yonutas HM, Crish SD, Sullivan PG, Darvesh AS, Brown CM, Richardson JR
ACS chemical neuroscience 2017 Dec 20;8(12):2759-2765

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MitoNEET Deficiency Alleviates Experimental Alcoholic Steatohepatitis in Mice by Stimulating Endocrine Adiponectin-Fgf15 Axis.

Hu X, Jogasuria A, Wang J, Kim C, Han Y, Shen H, Wu J, You M
The Journal of biological chemistry 2016 Oct 21;291(43):22482-22495

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TP53-inducible Glycolysis and Apoptosis Regulator (TIGAR) Metabolically Reprograms Carcinoma and Stromal Cells in Breast Cancer.

Ko YH, Domingo-Vidal M, Roche M, Lin Z, Whitaker-Menezes D, Seifert E, Capparelli C, Tuluc M, Birbe RC, Tassone P, Curry JM, Navarro-Sabaté À, Manzano A, Bartrons R, Caro J, Martinez-Outschoorn U
The Journal of biological chemistry 2016 Dec 16;291(51):26291-26303

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Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.

Lai YC, Kondapalli C, Lehneck R, Procter JB, Dill BD, Woodroof HI, Gourlay R, Peggie M, Macartney TJ, Corti O, Corvol JC, Campbell DG, Itzen A, Trost M, Muqit MM
The EMBO journal 2015 Nov 12;34(22):2840-61

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Defining roles of PARKIN and ubiquitin phosphorylation by PINK1 in mitochondrial quality control using a ubiquitin replacement strategy.

Ordureau A, Heo JM, Duda DM, Paulo JA, Olszewski JL, Yanishevski D, Rinehart J, Schulman BA, Harper JW
Proceedings of the National Academy of Sciences of the United States of America 2015 May 26;112(21):6637-42

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Leydig cell steroidogenesis unexpectedly escapes mitochondrial dysfunction in prematurely aging mice.

Shabalina IG, Landreh L, Edgar D, Hou M, Gibanova N, Atanassova N, Petrovic N, Hultenby K, Söder O, Nedergaard J, Svechnikov K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Aug;29(8):3274-86

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Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation.

Kazlauskaite A, Martínez-Torres RJ, Wilkie S, Kumar A, Peltier J, Gonzalez A, Johnson C, Zhang J, Hope AG, Peggie M, Trost M, van Aalten DM, Alessi DR, Prescott AR, Knebel A, Walden H, Muqit MM
EMBO reports 2015 Aug;16(8):939-54

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Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis.

Ordureau A, Sarraf SA, Duda DM, Heo JM, Jedrychowski MP, Sviderskiy VO, Olszewski JL, Koerber JT, Xie T, Beausoleil SA, Wells JA, Gygi SP, Schulman BA, Harper JW
Molecular cell 2014 Nov 6;56(3):360-375

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PINK1 rendered temperature sensitive by disease-associated and engineered mutations.

Narendra DP, Wang C, Youle RJ, Walker JE
Human molecular genetics 2013 Jul 1;22(13):2572-89

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PINK1 drives Parkin self-association and HECT-like E3 activity upstream of mitochondrial binding.

Lazarou M, Narendra DP, Jin SM, Tekle E, Banerjee S, Youle RJ
The Journal of cell biology 2013 Jan 21;200(2):163-72

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Isolation of sarcolemmal plasma membranes by mechanically skinning rat skeletal muscle fibers for phospholipid analysis.

Fajardo VA, McMeekin L, Basic A, Lamb GD, Murphy RM, LeBlanc PJ
Lipids 2013 Apr;48(4):421-30

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16006-1-AP(mitoNEET,CISD1 antibody) at dilution of 1:4000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CISD1 antibody 16006-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-mitoNEET,CISD1 (IP:16006-1-AP, 3ug; Detection:16006-1-AP 1:2000) with HepG2 cells lysate 600ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human heart using 16006-1-AP(mitoNEET,CISD1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The CISD1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CISD1. This antibody recognizes human,mouse,ratzebrafish antigen. The CISD1 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.

Appendix

16006-1-AP.pdf

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