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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP51153PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51153PU-N, RRID:AB_11143129
- Product name
- anti CXXC4 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 164-194aa) of human CXXC4.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of CXXC4 antibody Cat.-No. AP51153PU-N in mouse testis tissue lysates (35ug/lane).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in K562 cell line lysates (35ug/lane) using CXXC4 antibody Cat.-No. AP51153PU-N. This demonstrates the CXXC4 antibody detected the CXXC4 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human colon carcinoma reacted with CXXC4 antibody Cat.-No. AP51153PU-N (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using CXXC4 antibody Cat.-No. AP51153PU-N .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
