GTX124205

Zhou Y, Zheng X, Lu J, Chen W, Li X, Zhao L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(6):2548-2559

SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function.

Sun J, He X, Zhu Y, Ding Z, Dong H, Feng Y, Du J, Wang H, Wu X, Zhang L, Yu X, Lin A, McDonald T, Zhao D, Wu H, Hua WK, Zhang B, Feng L, Tohyama K, Bhatia R, Oberdoerffer P, Chung YJ, Aplan PD, Boultwood J, Pellagatti A, Khaled S, Kortylewski M, Pichiorri F, Kuo YH, Carlesso N, Marcucci G, Jin H, Li L
Cell stem cell 2018 Sep 6;23(3):355-369.e9

DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.

Chatterjee B, Lin MH, Chen CC, Peng KL, Wu MS, Tseng MC, Chen YJ, Shen CJ
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Nov;1861(11):1048-1061

TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.

Ni K, Dansranjavin T, Rogenhofer N, Oeztuerk N, Deuker J, Bergmann M, Schuppe HC, Wagenlehner F, Weidner W, Steger K, Schagdarsurengin U
Human reproduction (Oxford, England) 2016 Jul;31(7):1411-24

IGF2BP1 expression in human mesenchymal stem cells significantly affects their proliferation and is under the epigenetic control of TET1/2 demethylases.

Mahaira LG, Katsara O, Pappou E, Iliopoulou EG, Fortis S, Antsaklis A, Fotinopoulos P, Baxevanis CN, Papamichail M, Perez SA
Stem cells and development 2014 Oct 15;23(20):2501-12

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Supportive validation

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Main image
Experimental details: TET2 antibody [N2-2], N-term detects TET2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract5% SDS-PAGETET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

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Main image
Experimental details: TET2 antibody detects TET2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells5% SDS-PAGETET2 antibody (GTX124205) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

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Main image
Experimental details: TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET2 protein stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.

Supportive validation

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Main image
Experimental details: TET2 antibody [N2-2], N-term immunoprecipitates TET2 protein in IP experiments.IP samples: 30 £gg whole cell extract of TET2-transfected 293T cells.A. Control with 3 £gg of preimmune Rabbit IgGB. Immunoprecipitation of TET2 protein by 3 £gg TET2 antibody [N2-2], N-term (GTX124205)5 % SDS-PAGEThe immunoprecipitated TET2 protein was detected by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:3000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Supportive validation

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Main image
Experimental details: Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 ?g of TET2 antibody [N2-2], N-term (GTX124205).Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205).EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Supportive validation

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Main image
Experimental details: TET2 antibody [N2-2], N-term detects TET2 protein on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Supportive validation

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Main image
Experimental details: TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded cervical carcinoma. TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Supportive validation

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Main image
Experimental details: Cross-linked ChIP was performed with U2OS chromatin extract and 5 £gg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.