Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [2]
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Validation data
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- Product number
- PA5-78514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TET2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2, DDDDK-human TET2-transfected 293T
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of TET2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with TET2 polyclonal antibody (Product # PA5-78514) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TET2 Polyclonal Antibody (Product # PA5-78514). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 Polyclonal Antibody (Product # PA5-78514) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TET2 Polyclonal Antibody detects TET2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells.5% SDS-PAGE. TET2 Polyclonal Antibody (Product # PA5-78514) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TET2 Polyclonal Antibody detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 protein stained by TET2 Polyclonal Antibody (Product # PA5-78514) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of TET2 in U2OS chromatin extract using 5 µg of sample. Immunoprecipitation was performed using TET2 polyclonal antibody (Product # PA5-78514). Analysis of the precipitated DNA was done by PCR using primer targeting CCND2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with U2OS chromatin extract and 5 µg of either control rabbit IgG or TET2 Polyclonal Antibody (Product # PA5-78514). The precipitated DNA was detected by PCR with primer set targeting to CCND2.