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LearnPA5-85488
antibody from Invitrogen Antibodies
Targeting: TET2
FLJ20032, KIAA1546
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- PA5-85488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TET2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Positive Control: 293T, A431, HeLa, HepG2, DDDDK-human TET2-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.55 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TET2 Polyclonal Antibody detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 protein stained by TET2 Polyclonal Antibody (Product # PA5-85488) diluted at 1:1,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TET2 Polyclonal Antibody detects TET2 protein on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded cervical carcinoma. TET2 Polyclonal Antibody (Product # PA5-85488) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TET2 in paraffin-embedded breast cancer using TET2 polyclonal antibody (Product # PA5-85488) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TET2 Polyclonal Antibody detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 Polyclonal Antibody (Product # PA5-85488) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TET2 in paraffin-embedded cervical carcinoma using TET2 polyclonal antibody (Product # PA5-85488) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP (chromatin immunoprecipitation) analysis of TET2 in U2OS chromatin extract with TET2 polyclonal antibody (Product # PA5-85488) using 5 µg of sample.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with U2OS chromatin extract and 5 µg of either control rabbit IgG or TET2 Polyclonal Antibody (Product # PA5-85488). The precipitated DNA was detected by PCR with primer set targeting to CCND2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of TET2 in 293T whole cell extracts with TET2 polyclonal antibody (Product # PA5-85488) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
