Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 50-9882-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GARP Monoclonal Antibody (G14D9), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin). GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on the surface of activated Treg cells has been reported to be necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta.
- Antibody clone number
- G14D9
- Concentration
- 5 µL/Test
Submitted references Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.
Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.
Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg.
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells.
Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells.
Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression.
Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins.
The GARP gene encodes a new member of the family of leucine-rich repeat-containing proteins.
Carrillo-Galvez AB, Cobo M, Cuevas-Ocaña S, Gutiérrez-Guerrero A, Sánchez-Gilabert A, Bongarzone P, García-Pérez A, Muñoz P, Benabdellah K, Toscano MG, Martín F, Anderson P
Stem cells (Dayton, Ohio) 2015 Jan;33(1):183-95
Stem cells (Dayton, Ohio) 2015 Jan;33(1):183-95
Phenotypic and functional characteristics of CD4+ CD39+ FOXP3+ and CD4+ CD39+ FOXP3neg T-cell subsets in cancer patients.
Schuler PJ, Schilling B, Harasymczuk M, Hoffmann TK, Johnson J, Lang S, Whiteside TL
European journal of immunology 2012 Jul;42(7):1876-85
European journal of immunology 2012 Jul;42(7):1876-85
Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg.
Stockis J, Colau D, Coulie PG, Lucas S
European journal of immunology 2009 Dec;39(12):3315-22
European journal of immunology 2009 Dec;39(12):3315-22
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells.
Tran DQ, Andersson J, Wang R, Ramsey H, Unutmaz D, Shevach EM
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 11;106(32):13445-50
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 11;106(32):13445-50
Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells.
Wang R, Kozhaya L, Mercer F, Khaitan A, Fujii H, Unutmaz D
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 11;106(32):13439-44
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 11;106(32):13439-44
Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression.
Wang R, Wan Q, Kozhaya L, Fujii H, Unutmaz D
PloS one 2008 Jul 16;3(7):e2705
PloS one 2008 Jul 16;3(7):e2705
Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins.
Macaulay IC, Tijssen MR, Thijssen-Timmer DC, Gusnanto A, Steward M, Burns P, Langford CF, Ellis PD, Dudbridge F, Zwaginga JJ, Watkins NA, van der Schoot CE, Ouwehand WH
Blood 2007 Apr 15;109(8):3260-9
Blood 2007 Apr 15;109(8):3260-9
The GARP gene encodes a new member of the family of leucine-rich repeat-containing proteins.
Ollendorff V, Noguchi T, deLapeyriere O, Birnbaum D
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1994 Feb;5(2):213-9
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 1994 Feb;5(2):213-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stimulated with immobilized Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81), soluble Anti-Human CD28 Functional Grade Purified (Product # 16-0289-81), and Human IL-2 Recombinant Protein (Product # 14-8029-81) for 24 hours. Cells were surface stained with Anti-Human CD4 PE (Product # 12-0048-42) and Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or Anti-Human GARP eFluor® 660 (right). Cells were then fixed and permeabilized with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) followed by intracellular staining with Anti-Human Foxp3 PE (Product # 12-4776-42). Viable lymphocytes, as determined by Fixable Viability Dye eFluor® 450, were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 GARP binds LAP/TGF-beta1 to the surface of mASCs. (A): Murine ASCs were surface stained for GARP-PE and LAP-Alexa488 or isotype controls (bottom panels) as described in Materials and Methods. Stainings are shown separately (panels I and II) and overlapping (panel III). A cut mask representing colocalized GARP/LAP/TGF-beta1 is shown in panel IV. Cells were imaged using a Zeiss LSM 710 confocal microscope. (B): Murine ASCs were transduced with lentiviral vectors expressing a nonspecific shRNA (LV-CTRL) or GARP-specific shRNAs (LV#3 and LV#6) and GARP silencing in mASCs was assessed by qPCR (left panel) and flow cytometry (right panel). The general vector copy number/mASC for the different LVs used was two to three copies per cell. (C): LV-CTRL and LV#6 mASCs were stained for GARP (left panels) or GARP and LAP/TGF-beta1 (right panels). One representative experiment out of three is shown. (D): The LAP/TGF-beta1 expression levels (MFI) on NT, LV-CTRL, LV#3, and LV#6 mASCs were measured on a flow cytometer. Results are shown as mean (SEM) of three independent experiments. *, p < .05 versus LV-CTRL. Abbreviations: GARP, glycoprotein A repetitions predominant; LAP, latency-associated peptide; LV, lentiviral vector; TGF-beta1, transforming growth factor-beta1.