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- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-23301 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GARP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress.
Chaumonnot K, Masson S, Sikner H, Bouchard A, Baverel V, Bellaye PS, Collin B, Garrido C, Kohli E
Cell death & disease 2021 Jan 22;12(1):114
Cell death & disease 2021 Jan 22;12(1):114
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GARP in BALB/c splenocytes. Samples were incubated in GARP polyclonal antibody (Product # PA5-23301) followed by FITC conjugated secondary and anti-mouse CD4 PE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 GARP and CD11b expression on M1 and M2 macrophages. A FACS analysis of GARP membrane expression on M1 and M2 macrophages treated or not with Tg ( n = 4). B Kinetics of GARP expression on M1 and M2 macrophages treated or not with Tg for the indicated times ( n = 3). C GARP analysis by western-blot in M1 and M2 macrophages treated or not with Tg (a representative image is shown; n = 2). D CD11b ( n = 8) and CD18 ( n = 4) membrane expression on M1 and M2 macrophages treated or not with Tg (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001).
