20697-1-AP

antibody from Proteintech Group

Targeting: SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

Provider product page for 20697-1-AP

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Immunocytochemistry

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Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [67]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 20697-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20697-1-AP, RRID:AB_10694828
Product name: SHH antibody
Antibody type: Polyclonal
Description: SHH antibody (Cat. #20697-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SHH protein structure - 20697-1-AP shown in red.

Submitted references Targeted inhibition of the HNF1A/SHH axis by triptolide overcomes paclitaxel resistance in non-small cell lung cancer.

Li LB, Yang LX, Liu L, Liu FR, Li AH, Zhu YL, Wen H, Xue X, Tian ZX, Sun H, Li PC, Zhao XG
Acta pharmacologica Sinica 2024 May;45(5):1060-1076

Alginate microspheres encapsulating hox transcript antisense RNA siRNA regulate the Hedgehog-Gli1 pathway to alleviate epidermal growth factor receptor tyrosine kinase inhibitors resistance.

Lu G, Zhong H, Gao J, Zhang Y
Journal of biomaterials applications 2024 Mar;38(8):877-889

Ablated Sonic Hedgehog Signaling in the Dentate Gyrus of the Dorsal and Ventral Hippocampus Impairs Hippocampal-Dependent Memory Tasks and Emotion in a Rat Model of Depression.

Luo Y, Wang Y, Qiu F, Hou G, Liu J, Yang H, Wu M, Dong X, Guo D, Zhong Z, Zhang X, Ge J, Meng P
Molecular neurobiology 2024 Jul;61(7):4352-4368

Molecular mechanism of angiogenesis for cerebral infarction rats by acupuncture intervention based on sonic hedgehog signaling pathway.

Wu HN, Li J, He Y, Georgi R, Kolberg B, Wang SY
Physiology & behavior 2024 Feb 1;274:114420

Leonurine Regulates Hippocampal Nerve Regeneration in Rats with Chronic and Unpredictable Mild Stress by Activating SHH/GLI Signaling Pathway and Restoring Gut Microbiota and Microbial Metabolic Homeostasis.

Meng P, Zhang X, Li D, Yang H, Lin X, Zhao H, Li P, Wang Y, Wang X, Ge J
Neural plasticity 2023;2023:1455634

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway.

Mai L, He G, Chen J, Zhu J, Chen S, Yang H, Zhang M, Hou X, Ke M, Li X
Diabetes, metabolic syndrome and obesity : targets and therapy 2023;16:1731-1743

Itraconazole inhibits proliferation, induces apoptosis, and reduces angiogenesis of hemangioma endothelial cells by downregulating the hedgehog signaling pathway.

Dong J, Cui J, Shi X, Wang T, Liu S
Heliyon 2023 Sep;9(9):e19244

Rgl-exomiR-7972, a novel plant exosomal microRNA derived from fresh Rehmanniae Radix, ameliorated lipopolysaccharide-induced acute lung injury and gut dysbiosis.

Qiu FS, Wang JF, Guo MY, Li XJ, Shi CY, Wu F, Zhang HH, Ying HZ, Yu CH
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Sep;165:115007

Protective effect of benzaldehyde combined with albendazole against brain injury induced by Angiostrongylus cantonensis infection in mice.

Chen KY, Cheng CJ, Chen YJ, Chiu CH, Wang LC
International journal of antimicrobial agents 2023 Nov;62(5):106963

Diallyl trisulfide inhibits gastric cancer stem cell properties through ΔNp63/sonic hedgehog pathway.

Ge M, Zhu J, Yi K, Chen Y, Cao W, Wang M, Xie C, Li X, Geng S, Wu J, Zhong C, Cao H, Jiang Z, Han H
Molecular carcinogenesis 2023 Nov;62(11):1673-1685

Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway.

Cao WS, Zhao MJ, Chen Y, Zhu JY, Xie CF, Li XT, Geng SS, Zhong CY, Fu JY, Wu JS
Ecotoxicology and environmental safety 2023 Mar 1;252:114605

Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway.

He CH, Lv JM, Khan GJ, Duan H, Wang W, Zhai KF, Zou GA, Aisa HA
Phytotherapy research : PTR 2023 Jul;37(7):2745-2758

Distinct bulge stem cell populations maintain the pilosebaceous unit in a β-catenin-dependent manner.

Han J, Lin K, Choo H, Chen Y, Zhang X, Xu RH, Wang X, Wu Y
iScience 2023 Jan 20;26(1):105805

Superenhancer activation of KLHDC8A drives glioma ciliation and hedgehog signaling.

Lee D, Gimple RC, Wu X, Prager BC, Qiu Z, Wu Q, Daggubati V, Mariappan A, Gopalakrishnan J, Sarkisian MR, Raleigh DR, Rich JN
The Journal of clinical investigation 2023 Jan 17;133(2)

Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway.

Lin Q, Yu T, Li X, Lin X, Fan Y, Xu L
European journal of histochemistry : EJH 2023 Dec 12;67(4)

Senescent renal tubular epithelial cells activate fibroblasts by secreting Shh to promote the progression of diabetic kidney disease.

Wang D, Yin L, Chen R, Tan W, Liang L, Xiang J, Zhang H, Zhou X, Deng H, Guo B, Wang Y
Frontiers in medicine 2022;9:1018298

Resveratrol Induces Apoptosis, Suppresses Migration, and Invasion of Cervical Cancer Cells by Inhibiting the Hedgehog Signaling Pathway.

Jiang J, Liu Z, Zhou X, Peng F, Wang Z, Li F, Li M
BioMed research international 2022;2022:8453011

TLR4 Modulates Senescence and Paracrine Action in Placental Mesenchymal Stem Cells via Inhibiting Hedgehog Signaling Pathway in Preeclampsia.

Zhong Y, Zhang Y, Liu W, Zhao Y, Zou L, Liu X
Oxidative medicine and cellular longevity 2022;2022:7202837

Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.

Lyu X, Zhang X, Sun L, Wang J, Wang D
Computational intelligence and neuroscience 2022;2022:1529132

Study on the Mechanism of Sancao Tiaowei Decoction in the Treatment of MNNG-Induced Precancerous Lesions of Gastric Carcinoma Through Hedgehog Signaling Pathway.

Cai Y, Cao Y, Cheng S, Zou L, Yang T, Zhang Y, Shou Q, Chen B, Chen W
Frontiers in oncology 2022;12:841553

Ursolic acid inhibits the activation of smoothened-independent non-canonical hedgehog pathway in colorectal cancer by suppressing AKT signaling cascade.

Chen L, Liu M, Yang H, Ren S, Sun Q, Zhao H, Ming T, Tang S, Tao Q, Zeng S, Meng X, Xu H
Phytotherapy research : PTR 2022 Sep;36(9):3555-3570

Intact Fibroblast Growth Factor 23 Regulates Chronic Kidney Disease-Induced Myocardial Fibrosis by Activating the Sonic Hedgehog Signaling Pathway.

Li L, Gan H
Journal of the American Heart Association 2022 Sep 20;11(18):e026365

Hedgehog Signalling Contributes to Trauma-Induced Tendon Heterotopic Ossification and Regulates Osteogenesis through Antioxidant Pathway in Tendon-Derived Stem Cells.

Li G, Deng Y, Li K, Liu Y, Wang L, Wu Z, Chen C, Zhang K, Yu B
Antioxidants (Basel, Switzerland) 2022 Nov 16;11(11)

Metformin regulates macrophage polarization via the Shh signaling pathway to improve pulmonary vascular development in bronchopulmonary dysplasia.

Xiang X, Zhou L, Lin Z, Qu X, Chen Y, Xia H
IUBMB life 2022 Mar;74(3):259-271

Secretion of the disulfide bond generating catalyst QSOX1 from pancreatic tumor cells into the extracellular matrix: association with extracellular vesicles and matrix proteins.

Millar-Haskell CS, Sperduto JL, Slater JH, Thorpe C, Gleghorn JP
Journal of extracellular biology 2022 Jul;1(7)

Effect of kaempferol on hedgehog signaling pathway in rats with --chronic atrophic gastritis - Based on network pharmacological screening and experimental verification.

Tu W, Hong Y, Huang M, Chen M, Gan H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Jan;145:112451

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia.

Chen X, Sheng L, Ma J, Qi D, Li X, Wang Z, Wu Z, Wong L, Huang JH, Wu E, Ma Q, Zhang D
iScience 2022 Jan 21;25(1):103647

Maternal sevoflurane exposure affects neural stem cell differentiation in offspring rats through NRF2 signaling.

Zhang Y, Kuai S, Zhang Y, Xue H, Wu Z, Zhao P
Neurotoxicology 2022 Dec;93:348-354

BRD4 promotes the migration and invasion of bladder cancer cells through the Sonic hedgehog signaling pathway and enhances cisplatin resistance.

Liu T, Zhang Z, Wang C, Huang H, Li Y
Biochemistry and cell biology = Biochimie et biologie cellulaire 2022 Apr;100(2):179-187

Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.

Cao W, Li Y, Sun H, Yang C, Zhu J, Xie C, Li X, Wu J, Geng S, Wang L, Sun L, Geng G, Han H, Zhong C
Frontiers in cell and developmental biology 2021;9:679806

Resveratrol Inhibition of Renal Cancer Stem Cell Characteristics and Modulation of the Sonic Hedgehog Pathway.

Sun H, Zhang T, Liu R, Cao W, Zhang Z, Liu Z, Qian W, Wang D, Yu D, Zhong C
Nutrition and cancer 2021;73(7):1157-1167

Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition.

Guo K, Wang P, Zhang L, Zhou Y, Dai X, Yan Y, Chen Y, Wasan HS, Yu J, Ruan S, Sun L
Cancer science 2021 Oct;112(10):4176-4186

Differential effects of EPA and DHA on DSS-induced colitis in mice and possible mechanisms involved.

Zhang Z, Xue Z, Yang H, Zhao F, Liu C, Chen J, Lu S, Zou Z, Zhou Y, Zhang X
Food & function 2021 Mar 1;12(4):1803-1817

ActivinA activates Notch1-Shh signaling to regulate proliferation in C2C12 skeletal muscle cells.

Ma L, Li C, Lian S, Xu B, Yuan J, Lu J, Yang H, Guo J, Ji H
Molecular and cellular endocrinology 2021 Jan 1;519:111055

Benzidine promotes the stemness of bladder cancer stem cells via activation of the Sonic hedgehog pathway.

Wang D, Xie D, Bi L, Wang Y, Zou C, Chen L, Geng H, Qian W, Li Y, Sun H, Wang X, Lu Y, Yu D, Zhong C
Oncology letters 2021 Feb;21(2):146

Repair of spinal cord injury in rats via exosomes from bone mesenchymal stem cells requires sonic hedgehog.

Jia Y, Yang J, Lu T, Pu X, Chen Q, Ji L, Luo C
Regenerative therapy 2021 Dec;18:309-315

All-trans-retinoic acid suppresses rat embryo hindlimb bud mesenchymal chondrogenesis by modulating HoxD9 expression.

Hong Q, Li XD, Xie P, Du SX
Bioengineered 2021 Dec;12(1):3900-3911

Procyanidin B2 alleviates liver injury caused by cold stimulation through Sonic hedgehog signalling and autophagy.

Ma L, Li C, Lian S, Xu B, Lv H, Liu Y, Lu J, Ji H, Li S, Guo J, Yang H
Journal of cellular and molecular medicine 2021 Aug;25(16):8015-8027

Exosomes secreted from sonic hedgehog-modified bone mesenchymal stem cells facilitate the repair of rat spinal cord injuries.

Jia Y, Lu T, Chen Q, Pu X, Ji L, Yang J, Luo C
Acta neurochirurgica 2021 Aug;163(8):2297-2306

Neuroprotective Effects of the Sonic Hedgehog Signaling Pathway in Ischemic Injury through Promotion of Synaptic and Neuronal Health.

Yin S, Bai X, Xin D, Li T, Chu X, Ke H, Han M, Chen W, Li X, Wang Z
Neural plasticity 2020;2020:8815195

Cigarette smoke supports stemness and epithelial-mesenchymal transition in bladder cancer stem cells through SHH signaling.

Sun X, Song J, Li E, Geng H, Li Y, Yu D, Zhong C
International journal of clinical and experimental pathology 2020;13(6):1333-1348

Purmorphamine Attenuates Neuro-Inflammation and Synaptic Impairments After Hypoxic-Ischemic Injury in Neonatal Mice via Shh Signaling.

Liu D, Bai X, Ma W, Xin D, Chu X, Yuan H, Qiu J, Ke H, Yin S, Chen W, Wang Z
Frontiers in pharmacology 2020;11:204

Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.

Ge W, Tan SJ, Wang SH, Li L, Sun XF, Shen W, Wang X
Theranostics 2020;10(17):7581-7598

Matrix metalloproteinase-9 regulates the blood brain barrier via the hedgehog pathway in a rat model of traumatic brain injury.

Wu MY, Gao F, Yang XM, Qin X, Chen GZ, Li D, Dang BQ, Chen G
Brain research 2020 Jan 15;1727:146553

Liraglutide regulates proliferation, differentiation, and apoptosis of preosteoblasts through a signaling network of Notch/Wnt/Hedgehog signaling pathways.

Hou HW, Xue P, Wang Y, Li YK
European review for medical and pharmacological sciences 2020 Dec;24(23):12408-12422

An SGLT2 inhibitor modulates SHH expression by activating AMPK to inhibit the migration and induce the apoptosis of cervical carcinoma cells.

Xie Z, Wang F, Lin L, Duan S, Liu X, Li X, Li T, Xue M, Cheng Y, Ren H, Zhu Y
Cancer letters 2020 Dec 28;495:200-210

Exosomal Sonic Hedgehog derived from cancer-associated fibroblasts promotes proliferation and migration of esophageal squamous cell carcinoma.

Zhao G, Li H, Guo Q, Zhou A, Wang X, Li P, Zhang S
Cancer medicine 2020 Apr;9(7):2500-2513

Histone H1 improves regeneration after mouse spinal cord injury and changes shape and gene expression of cultured astrocytes.

Kleene R, Loers G, Jakovcevski I, Mishra B, Schachner M
Restorative neurology and neuroscience 2019;37(4):291-313

Anti-apoptotic effect of the Shh signaling pathway in cigarette smoke extract induced MLE 12 apoptosis.

Li J, Zong D, Chen Y, Chen P
Tobacco induced diseases 2019;17:49

Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells.

Li E, Zhang T, Sun X, Li Y, Geng H, Yu D, Zhong C
Oncology letters 2019 Sep;18(3):3081-3091

Genistein inhibits nasopharyngeal cancer stem cells through sonic hedgehog signaling.

Zhang Q, Cao WS, Wang XQ, Zhang M, Lu XM, Chen JQ, Chen Y, Ge MM, Zhong CY, Han HY
Phytotherapy research : PTR 2019 Oct;33(10):2783-2791

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Zhu R, Gires O, Zhu L, Liu J, Li J, Yang H, Ju G, Huang J, Ge W, Chen Y, Lu Z, Wang H
Nature communications 2019 Jun 28;10(1):2863

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Ge M, Zhang L, Cao L, Xie C, Li X, Li Y, Meng Y, Chen Y, Wang X, Chen J, Zhang Q, Shao J, Zhong C
International journal of food sciences and nutrition 2019 Aug;70(5):570-578

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways.

Zhang H, Nan W, Wang S, Song X, Si H, Li T, Li G
Frontiers in pharmacology 2018;9:674

Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway.

Qian W, Kong X, Zhang T, Wang D, Song J, Li Y, Li X, Geng H, Min J, Kong Q, Liu J, Liu Z, Wang D, Zhang Z, Yu D, Zhong C
Oncogenesis 2018 Mar 13;7(3):24

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Jia W, Jiang X, Liu W, Wang L, Zhu B, Zhu H, Liu X, Zhong M, Xie D, Huang W, Jia W, Li S, Liu X, Zuo X, Cheng D, Dai J, Ren C
International journal of oncology 2018 Jun;52(6):1787-1800

Low-intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling.

Matsumoto K, Shimo T, Kurio N, Okui T, Ibaragi S, Kunisada Y, Obata K, Masui M, Pai P, Horikiri Y, Yamanaka N, Takigawa M, Sasaki A
Journal of cellular biochemistry 2018 Jun;119(6):4352-4360

CCL2/CCR2 axis induces hepatocellular carcinoma invasion and epithelial-mesenchymal transition in vitro through activation of the Hedgehog pathway.

Zhuang H, Cao G, Kou C, Liu T
Oncology reports 2018 Jan;39(1):21-30

Different effects of dexmedetomidine and midazolam on the expression of NR2B and GABAA-α1 following peripheral nerve injury in rats.

Chen J, Li H, Lim G, McCabe MF, Zhao W, Yang Y, Ma W, Li N
IUBMB life 2018 Feb;70(2):143-152

NRF2 Is a Potential Modulator of Hyperresistance to Arsenic Toxicity in Stem-Like Keratinocytes.

Wu X, Yang B, Hu Y, Sun R, Wang H, Fu J, Hou Y, Pi J, Xu Y
Oxidative medicine and cellular longevity 2017;2017:7417694

Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.

Wang D, Kong X, Li Y, Qian W, Ma J, Wang D, Yu D, Zhong C
Biochemical and biophysical research communications 2017 Nov 4;493(1):521-527

Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.

Zhu JY, Yang X, Chen Y, Jiang Y, Wang SJ, Li Y, Wang XQ, Meng Y, Zhu MM, Ma X, Huang C, Wu R, Xie CF, Li XT, Geng SS, Wu JS, Zhong CY, Han HY
Phytotherapy research : PTR 2017 Apr;31(4):680-688

Dicer1 Ablation Impairs Responsiveness of Cerebellar Granule Neuron Precursors to Sonic Hedgehog and Disrupts Expression of Distinct Cell Cycle Regulator Genes.

Liu Q, Jiang M, Kuang Y, Shu X, Li J, Li MW, Li H
Cerebellum (London, England) 2017 Apr;16(2):450-461

Neuroprotective Effects of a Smoothened Receptor Agonist against Early Brain Injury after Experimental Subarachnoid Hemorrhage in Rats.

Hu Q, Li T, Wang L, Xie Y, Liu S, Bai X, Zhang T, Bo S, Xin D, Xue H, Li G, Wang Z
Frontiers in cellular neuroscience 2016;10:306

High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation.

Su C, Zhang B, Liu W, Zheng H, Sun L, Tong J, Wang T, Jiang X, Liang H, Xue L, Zhang Q
Oncology reports 2016 Aug;36(2):1048-54

Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction.

Pang P, Shimo T, Takada H, Matsumoto K, Yoshioka N, Ibaragi S, Sasaki A
Biochemical and biophysical research communications 2015 Nov 6;467(1):21-6

Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro.

Lu J, Lu K, Li D
Neural regeneration research 2012 Aug 5;7(22):1688-94

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse liver tissue were subjected to SDS PAGE followed by western blot with 20697-1-AP(SHH antibody) at dilution of 1:800
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using SHH antibody 20697-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line