F53068

antibody from NSJ Bioreagents

Targeting: SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F53068

Provider

NSJ Bioreagents

Product name

Shh Antibody / Sonic Hedgehog

Antibody type

Polyclonal

Description

This highly specific Shh antibody is suitable for use in Flow cytometry/Western blot/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Shh antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from mouse liver tissue lysate using Shh antibody. Predicted molecular weight: 45/27/19 kDa (1)

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using Shh antibody (green) compared to an isotype control of rabbit IgG (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.