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LearnMA5-41159
antibody from Invitrogen Antibodies
Targeting: SHH
HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- MA5-41159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHH Recombinant Rabbit Monoclonal Antibody (SC05-28)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SC05-28
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SHH using SHH Recombinant Monoclonal Antibody (Product # MA5-41159) at a dilution of 1:1,000. Lane 1: Hela , Lane 2: HepG2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SHH in Hela cells (green) using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SHH in A549 cells (green) using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of SHH in 293 cells (green) using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SHH in paraffin-embedded human liver cancer tissue using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SHH in paraffin-embedded human kidney tissue using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SHH in paraffin-embedded rat kidney tissue using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SHH in paraffin-embedded rat liver tissue using SHH Recombinant Monoclonal Antibody (Product # MA5-41159). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of SHH in Hela cells. The cells were fixed, permeabilized and stained with SHH Recombinant Monoclonal Antibody (Product # MA5-41159) at a dilution of 1:50 (red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. Unlabelled control (cells without incubation with primary antibody; black).
