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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF464 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Sonic Hedgehog/Shh N-Terminus Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Sonic Hedgehog/Shh N-Terminus in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) Dhh N-Terminus and rmIhh N-Terminus is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q62226- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib.
Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling.
Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells.
Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice.
Transcription factor 7-like 1 is involved in hypothalamo-pituitary axis development in mice and humans.
Heparan sulfate-modulated, metalloprotease-mediated sonic hedgehog release from producing cells.
Involvement of the Edar signaling in the control of hair follicle involution (catagen).
Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog.
Sonic hedgehog exerts distinct, stage-specific effects on tongue and taste papilla development.
Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
Kumari A, Yokota Y, Li L, Bradley RM, Mistretta CM
Scientific reports 2018 Nov 1;8(1):16150
Scientific reports 2018 Nov 1;8(1):16150
Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling.
Ermilov AN, Kumari A, Li L, Joiner AM, Grachtchouk MA, Allen BL, Dlugosz AA, Mistretta CM
PLoS genetics 2016 Nov;12(11):e1006442
PLoS genetics 2016 Nov;12(11):e1006442
Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells.
Zhang Z, Li Z, Deng W, He Q, Wang Q, Shi W, Chen Q, Yang W, Spector M, Gong A, Yu J, Xu X
Biochemical and biophysical research communications 2016 Nov 25;480(4):727-733
Biochemical and biophysical research communications 2016 Nov 25;480(4):727-733
Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice.
Singh VB, Singh MV, Gorantla S, Poluektova LY, Maggirwar SB
Scientific reports 2016 May 31;6:26876
Scientific reports 2016 May 31;6:26876
Transcription factor 7-like 1 is involved in hypothalamo-pituitary axis development in mice and humans.
Gaston-Massuet C, McCabe MJ, Scagliotti V, Young RM, Carreno G, Gregory LC, Jayakody SA, Pozzi S, Gualtieri A, Basu B, Koniordou M, Wu CI, Bancalari RE, Rahikkala E, Veijola R, Lopponen T, Graziola F, Turton J, Signore M, Mousavy Gharavy SN, Charolidi N, Sokol SY, Andoniadou CL, Wilson SW, Merrill BJ, Dattani MT, Martinez-Barbera JP
Proceedings of the National Academy of Sciences of the United States of America 2016 Feb 2;113(5):E548-57
Proceedings of the National Academy of Sciences of the United States of America 2016 Feb 2;113(5):E548-57
Heparan sulfate-modulated, metalloprotease-mediated sonic hedgehog release from producing cells.
Dierker T, Dreier R, Petersen A, Bordych C, Grobe K
The Journal of biological chemistry 2009 Mar 20;284(12):8013-22
The Journal of biological chemistry 2009 Mar 20;284(12):8013-22
Involvement of the Edar signaling in the control of hair follicle involution (catagen).
Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
The American journal of pathology 2006 Dec;169(6):2075-84
The American journal of pathology 2006 Dec;169(6):2075-84
Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog.
Rafuse VF, Soundararajan P, Leopold C, Robertson HA
Neuroscience 2005;131(4):899-916
Neuroscience 2005;131(4):899-916
Sonic hedgehog exerts distinct, stage-specific effects on tongue and taste papilla development.
Liu HX, Maccallum DK, Edwards C, Gaffield W, Mistretta CM
Developmental biology 2004 Dec 15;276(2):280-300
Developmental biology 2004 Dec 15;276(2):280-300
Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
Mistretta CM, Liu HX, Gaffield W, MacCallum DK
Developmental biology 2003 Feb 1;254(1):1-18
Developmental biology 2003 Feb 1;254(1):1-18
Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
Mistretta CM, Liu HX, Gaffield W, MacCallum DK
Developmental biology 2003 Feb 1;254(1):1-18
Developmental biology 2003 Feb 1;254(1):1-18
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
Gastroenterology 2001 Aug;121(2):317-28
Gastroenterology 2001 Aug;121(2):317-28
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
Gastroenterology 2001 Aug;121(2):317-28
Gastroenterology 2001 Aug;121(2):317-28
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human stomach tissue and mouse stomach tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF464) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Sonic Hedgehog/Shh in Mouse Embryo. Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF464) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
