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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-71641 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SPECC1L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.48 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SPECC1 in U-251MG cells using confocal microscopy. Samples were incubated with SPECC1 H1 polyclonal antibody (Product # PA5-71641) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) as a secondary antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPECC1 in paraffin-embedded human brain tissue. Samples were incubated with SPECC1 polyclonal antibody (Product # PA5-71641) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SPECC1 in HL-60 cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with SPECC1 polyclonal antibody (Product # PA5-71641) followed by a FITC conjugated goat anti-rabbit secondary antibody.
