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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Flow cytometry [1]
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- Product number
- F51246 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MICA Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific MICA antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the MICA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: Daudi ; 3: A431; 4: MCF-7; 5: U-87MG; 6: THP-1; Predicted molecular weight: 38-62 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MICA antibody at 1:2000 dilution. Lane 1: U-87 MG lysate; 2: A431; 3: Jurkat; Predicted molecular weight: 38-62 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of MICA antibody at 1:1000 dilution + HeLa lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SK-BR-3 cells using MICA antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary.
