Explore
Validate
Learn
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-80811 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MICA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human MICA.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MICA Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 2 µL with MICA Polyclonal Antibody (Product # PA5-80811) and 15 µL of 50 % Protein G agarose. Primary antibody: MICA Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human MICA in human breast-carcinoma with MICA Polyclonal Antibody (Product # PA5-80811, 1:1,000, formalin-fixed paraffin embedded sections). Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MICA Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 2 µL with MICA Polyclonal Antibody (Product # PA5-80811) and 15 µL of 50 % Protein G agarose. Primary antibody: MICA Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.
