H00008900-M02

antibody from Abnova Corporation

Targeting: CCNA1 CT146

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008900-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008900-M02, RRID:AB_913443

Product name

CCNA1 monoclonal antibody (M02), clone 4A11-5B5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CCNA1.

Antigen sequence

METGFPAIMYPGSFIGGWGEEYLSWEGPGLPDFVF
QQPVESEAMHCSNPKSGVVLATVARGPDACQILTR
APLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRC
YSGSENAFPPAGKKALPDCGVQEPPKQGFDIYMDE
LEQGDRDSCSVREGMAFEDVYEVDTGTLKSDLHFL
LDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEY
AEEIYQYLREAEIRHRPKAHYMKKQPDITEGMRTI
LVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSV
LRGKLQLVGTAAMLLASKYEEIYPPEVDEFVYITD
DTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQY
LRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSL
IAAAAFCLANYTVNKHFWPETLAAFTGYSLSEIVP
CLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLM
EPPAVLLLQ


Isotype

IgG

Antibody clone number

4A11-5B5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CCNA1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PTK2 and CCNA1. HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-CCNA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)