PA5-80414

antibody from Invitrogen Antibodies

Targeting: CCNA1 CT146

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80414

Provider

Invitrogen Antibodies

Product name

Cyclin A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human Cyclin A1/CCNA1.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cyclin A1 Immunoprecipitation using: Lane A: 0.5 mg THP1 Whole Cell Lysate 0.5 µL with Cyclin A1 Polyclonal Antibody (Product # PA5-80414) and 15 µL of 50 % Protein G agarose. Primary antibody: Cyclin A1 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.